Gene mutation key means that higher animals, lower animals suffer from the efficacy of a variety of elements, gene changes, including a single base, several base changes, the application of microfluidic microarray can test gene mutations, such as Hidradenia cryptica cwn-1 gene mutation, circulating tumor stem cell gene mutation. In general, all etiological genes are slowly replaced by replicative mutations containing polypeptide chain conformational polymorphism, bound wonderful fragment length polymorphism, in which the key to polypeptide chain conformational polymorphism is the occurrence of individual base differences in equal length DNA polypeptide chains, followed by conformational differences because of the efficacy of each other within the chain, applying fluorescent dye marker primer design, applying microfluidic chip to dissect the source of breast cancer infection The microfluidic chip can test the peptide chain conformational polymorphism of breast cancer infectious genes within 2 min, which is significantly less than the time required for capillary electrophoresis; mandatory fragment length polymorphism means that some unique point mutations can promote the lack of some non-specific bound endonuclease structural domains, and the microfluidic chip can detect and analyze the bound wonderful fragment length polymorphism. Overseas scientific research materials show that non-small cell lung cancer test products carry out certified scientific research testing for mutations in the ectodermal growth factor protein kinase (EGFR) gene can be carried out using microfluidic chips, according to a rapid and dexterous microfluidic chip technology that can detect EGFR quickly and at a cheap cost.
Association of microfluidic chip and gene precision transcriptome sequencing
The key to gene sequencing is to choose an excellent way to carry out specialized, standardized and rapid analysis of nucleotide coding sequences of higher and lower animals, and the list of works necessary for this whole process is especially great. At the present stage, the microfluidic chip laboratory key uses 96 array capillary electrophoresis to carry out specialized measurement of gene coding sequences, although at a certain level to accelerate the new project of people’s genome, but it can not complete the basic characteristics of high efficiency, dexterity, rapid, high quality and low cost, fully automatic and accurate, while the microfluidic chip can complete high efficiency gene sequencing, such as gut microbial gene sequencing, yeast Gene sequencing, liver cancer tumor stem cell gene sequencing and other tests are very high efficiency.
Association of microfluidic chip with high frequency increase of genes
Gene high frequency increase generally refers to the selection of polymerase chain reaction (PCR) to raise the overall target DNA fragments in total and to identify, separate and dissect the overall target DNA fragments according to the principle of electrophoresis, which requires more experimental reagents, long heating time, long cooling time and other defects. At the present stage, microfluidic chips can be used to increase the frequency of genes in the laboratory, and according to the fusion of microfluidic chips and PCR, a miniaturized microfluidic chip-PCR reflection management system has been created for this purpose, which may significantly get rid of the defects of PCR including PCR must more experimental reagents, long heating time, long cooling time, etc. Meanwhile, the microfluidic chip-PCR reflection management system increases the substance It is very easy to separate from the test article and can complete the test automation technology, integration and miniaturization. At this stage, the microfluidic chip-PCR reflection management system can detect soil pathogen genes, tumor stem cell genes, blood night gene, etc., which shows that its application is very broad.
In summary, microfluidic chip can be used in medicine gene diagnosis level, such as gene polymorphism test, gene precision transcriptome sequencing, gene high frequency sex increase, etc., so it can be seen that microfluidic chip and gene diagnosis are closely related, inferred that the application of microfluidic chip will be one of the new ways of gene diagnosis test analysis in the future.